Effect of mitochondrial ATP sensitive potassium channels opening on translocation of protein kinase C epsilon in cultured adult rat ventricular myocytes

AIM: To investigate the effects of mitochondrial ATP sensitive potassium (MitoKATP) channel opening on translocation of protein kinase C epsilon (PKCε) and the relationship between the translocation of PKCε and the production of reactive oxygen species. METHODS: The expression of PKCε in cultured adult rat ventricular myocytes was investigated with immunofluorescence and Western blotting techniques. RESULTS: （1） Diazoxide, a selective MitoKATP channel opener, caused a significant translocation to myofibrillar-like structures in cultured adult rat ventricular myocytes. (2) The N-2-mercaptopropionylglycine (MPG), a free radical scavenger, partly inhibited the translocation of PKCε caused by diazoxide. (3) Chelerythrine, a selective protein kinase C (PKC) inhibitor, completely blocked the translocation of PKCε caused by diazoxide. CONCLUSION: Opening of MitoKATP channel might activate PKCε and make it translocation to myofibrillar-like structures. PKCε activation occurs downstream of MitoKATP channel, and might be caused by production of reactive oxygen species after opening of MitoKATP channel.     

冷冻法测定梨的石细胞含量

以南果、安梨、砀山酥、秋白、锦丰、五九香、巴哈胜利、丰水、黄金、黄冠等10个梨品种果实为试材,对梨石细胞含量冷冻处理测定法的果肉切块大小、冷冻处理时间、匀浆时间、漂洗方法、平行性与准确度等进行了优化和系统研究,提出最适测定条件为果肉切块大小1.0cm3、-16～20℃冷冻处理24h、22000r/min匀浆3min。在此条件下,石细胞平均回收率为85.1%～94.1%,方法误差为4.65%～11.56%。研究结果为制定科学、实用的梨石细胞含量测定方法农业行业标准奠定了基础。     

爱拔益加肉鸡和北京油鸡体液免疫及细胞免疫功能的比较研究

试验选用1日龄健康爱拔益加(AA)肉鸡和北京油鸡公雏各72只。检测新城疫抗体效价,牛血清白蛋白抗体水平,外周血T、B淋巴细胞转化率以及T淋巴细胞亚群等指标。结果表明:北京油鸡二免后第7天的新城疫抗体效价和二免后第6天的牛血清白蛋白抗体水平都比AA肉鸡的高,但均无显著性差异(P>0.05)。北京油鸡的脂多糖(LPS)刺激指数始终比AA肉鸡的高(P>0.05);21日龄时,北京油鸡的刀豆素(A ConA)刺激指数、CD4+、CD8+T淋巴细胞数量以及CD4+/CD8+都比AA肉鸡的高,但均无显著性差异(P>0.05);而42日龄时,AA肉鸡的ConA刺激指数、CD4+、CD8+T淋巴细胞数量以及CD4+/CD8+却高于北京油鸡(P<0.05)。本试验结果提示,AA肉鸡和北京油鸡的体液免疫和细胞免疫功能之间存在一定的差异,但差异不显著。     

Comparative phosphoproteome analysis of cardiomyocytes preconditioned by diazoxide

AIM: To analyze and identify the phosphoproteins associated with diazoxide preconditioning. METHODS: Proteomics technique was used to investigate the changes of phosphoprotein after diazoxide preconditioning. Adult rat ventricular myocytes were pretreated in the presence and absence of 200 μmol/L diazoxide for 10 min. Phosphoproteins prepared and enriched respectively from control and diazoxide pretreated groups were then separated by two-dimensional (2D) gel electrophoresis and stained with sliver staining kit. Phosphoproteins of interest were further identified by mass spectrometry. RESULTS: Associated with diazoxide preconditioning, the proteins of chaperonin containing TCP-1 and hypothetical protein XP_346548 were phosphorylated significantly. The proteins of 94 kD glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphorylated markedly (P<0.05). CONCLUSION: These findings suggest that cardiomyocytes undergo significant posttranslational modification via phosphorylation in a multitude of proteins in response to diazoxide preconditioned signaling, which may mediate myocardioprotection signaling downstream mitochondrial staining dish K_ATP channel induced by ischemic preconditioning.     

辣椒品种的聚类及主成分分析

以辣椒为试材,对57份辣椒材料的12个农艺性状进行了聚类分析,对6个数量性状进行了主成分分析,旨在为辣椒资源分类以及亲本筛选提供依据。结果表明:聚类分析将57份辣椒材料划分为四大类群,单果质量成为影响聚类结果的最重要因素;主成分分析结果表明,前3个主成分就可以表达原6个数量性状所表达的遗传特性。     

黄瓜病害特点及防治

为减少保护地黄瓜的病害发生,介绍黄瓜靶斑病、炭疽病、霜霉病、角斑病的症状、病原菌及发病特点,从品种选择、种子处理、化学防治等方面,探讨上述病害的综合防治措施.     

杉木树干材积分析

就杉木人工林立木树干材积的估测，材积按树高分布，材积的组成等有关问题进行了探讨。     

棉花新品种赣棉杂1号的选育及示范

赣棉杂1号是江西省棉花研究所采用杂种优势利用的方法选育出的江西省首个优质、高产杂交棉新品种.该品种的母本为优质、抗病、高产的自育材料YL168;父本为经航天诱变育种材料中系统选育而成、适纺60支纱以上的特优材料YM-1;通过在F1代优势测定试验、品种比较试验、省级区域试验、生产示范等试验示范,赣棉杂1号表现突出,一般子棉产量为4 500 kg/hm2、皮棉产量为1 900 kg/hm2,霜前花率85%;2.5%跨长≥30 mm,比强≥33.2 cN/tex,马克隆值3.7～4.8;抗棉枯萎病、枯萎病指7.4,抗逆性能较强,综合性状优良.根据赣棉杂1号的特征特性提出了相应的高产栽培措施.     

国民党执政大陆时期腐败现象的法理透视

权力的集权设置与运行、法制失控是国民党执政大陆时期腐败愈演愈烈的法律原因.国民党的贪必腐、腐必败的历史,反映了腐败消解国家统一意志和前进动力、导致社会灾难和政权倾覆的不变真理.能否有效地抑制和清除腐败现象,决定着一个执政党执政的成败, 也决定着社会能否长治久安.今人当从国民党盛衰演变的历史中认真吸取教训.     

一维土壤入渗特性数值模拟

以非饱和土壤水分运动理论基础,利用Richards模型模拟不同实验处理下的一维垂直入渗过程,采用SWMS-1D软件对模型求解,用试验资料对模拟的累积入渗量与湿润锋运移距离结果进行了对比验证,二者基本吻合。对模拟结果分析得出,土壤质地和容重对一维垂直入渗累积入渗量、入渗速率和湿润锋运移距离影响较大,土壤初始含水率和入渗水头对其影响较小;并分别建立了不同土壤质地下的累积入渗量、入渗速率和湿润锋运移距离与入渗历时单参数幂函数计算式。